Immunological and chemical analyses revealed that Ibf1 mutants also showed changes to other cell wall polymers. Liquid chromatography-mass spectrometry profiling showed large modifications in the oligolignol pool of Ibf1 innerand outer-stem tissues that could be related to ectopie lignification. Chemical and NMR analyses indicated that bast fiber ectopie lignin was highly condensed and rich in G-units. As a proof of concept, we characterized the Ibf1 mutant and showed that the lignin content increased by 350% in outer stem tissues containing bast fibers but was unchanged in inner stem tissues containing xylem. We named this core collection the Linum usitatissimum (flax) Ibf mutants for lignified bast fibers and believe that this population represents a novel biological resource for investigating how bast fiber plants regulate lignin biosynthesis. Histochemical screening of a flax ethyl methanesulfonate population led to the identification of 93 independent M2 mutant families showing ectopie lignification in the secondary cell wall of stem bast fibers.
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